phosphorylated irs 1  (Cell Signaling Technology Inc)

Journal: eNeuro

Article Title: AD-Like Neuropsychiatric Dysfunction in a Mice Model Induced by a Combination of High-Fat Diet and Intraperitoneal Injection of Streptozotocin

doi: 10.1523/ENEURO.0310-24.2024

Figure Lengend Snippet: Combination of HFD and STZ/I.P. induced the dysregulation of the IRS1/AKT/ERK signaling pathway in mice. A , The typical graph of IRS1/AKT/ERK signaling pathway proteins in the hippocampus and PFC of mice. B , The statistical analysis of the Western blotting results in the hippocampus. C , The statistical analysis of the Western blotting results in the PFC. The data are presented as the mean ± SEM, with n = 3 in each group. * p < 0.05 and ** p < 0.01 compared with the Con group.

Article Snippet: Equal amounts of protein were separated on 10% SDS–PAGE gels, transferred onto polyvinylidene difluoride (PVDF) membranes, and incubated overnight at 4°C with the relevant primary antibodies: anti-phosphorylated AKT (p-AKT) and total AKT (1:1,000; Zen-Bio), anti-phosphorylated ERK (p-ERK) and total ERK (1:1,000; Santa Cruz Biotechnology), anti-phosphorylated IRS1 (p-IRS1) and total IRS1 (1:1,000; Abcam), anti-APP(1:1,000; Proteintech), anti-phosphorylated Tau (p-Tau) and total tau (1:1,000; Santa Cruz Biotechnology), anti-TREM1 and TREM2 (1:1,000; Abcam), and anti-β-actin (1:1,000; Zhongshan Biotechnology).

Techniques: Western Blot

Journal: Annals of Clinical and Translational Neurology

Article Title: Associations of renin–angiotensin system inhibitor use with brain insulin signaling and neuropathology

doi: 10.1002/acn3.52132

Figure Lengend Snippet: Associations of RAS inhibitor use with brain insulin signaling measures.

Article Snippet: Total IRS1 and phosphorylated IRS1 at Ser307 (pS 307 IRS1), and total AKT1 and phosphorylated AKT1 at Thr308 (pT 308 IRS1), were quantified using PathScan enzyme‐linked immunosorbent assay (ELISA) kits (Cell Signaling Technology, Danvers, MA) based on the traditional solid‐phase sandwich ELISA method.

Techniques: